BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube, Each. BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube. Remarks: Glucose – Any degree of yellow is positive. Acid end products from glucose fermentation turn the pH indicator from red (alkaline) to yellow (acid). of a Multitest System (Enterotube) for Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification . (ii) Modified lysine-lactose me- dium: a .. Bergey’s manual of determinative bacteriology.

Author: Zololkis Digal
Country: Nicaragua
Language: English (Spanish)
Genre: Science
Published (Last): 6 February 2007
Pages: 483
PDF File Size: 6.25 Mb
ePub File Size: 2.44 Mb
ISBN: 717-9-19537-712-3
Downloads: 60933
Price: Free* [*Free Regsitration Required]
Uploader: Mudal

H Edwards and Ewing s identification of Enterobacteriaceae. This test is mandatory if the database with VP is chosen. Any degree of blue should be considered positive. After completing this project the students should be able to:.

Biol Lab Manual: Interpretation of Enterotube II

System for the identification of Gram negative, oxidase positive bacteria. Identification of Unknown Organic Compounds Introduction The identification and characterization of engerotube structures of unknown substances are an important part of organic chemistry. Culture media are used for recognition and identification diagnosis of microorganisms.

Once we have obtained any growth, work flow in the Microbiology. Ancillary culture media, reagents and laboratory equipment as described.

Identify descriptive features More information. This test is helpful in identifying and differentiating species of Bacillus, Clostridium, Proteus, More information.

Metabolism sum of all chemical processes More information. American Society for Microbiology, Washington, D. If the database without VP is chosen, it may be necessary to perform the VP reaction as a supplemental test for selected organisms. Bergey s Manual of Determinative Bacteriology All of the guiee will fall into the following groups in Bergey’s Manual of Determinative Bacteriology The pink book on the shelf in the laboratory. To study chemical and physical properties of proteins from natural sources egg and milk and some chemical reactions of amino acid residues in these proteins.


The urease test is strongly positive for Proteus species and may be evident as early as 4 to 6 h after incubation; interprretation is weakly positive light pink color after 18 to 24 h incubation for Klebsiella and Enterobacter interpertation.

Level 1 Training Course. Gelatin Hydrolysis Test Protocol The Gelatin Hydrolysis Test is used to detect the ability of interpretafion to produce ebterotube enzyme gelatinase. Introduction Interprdtation we pick our culture from the incubator, we will have a grown culture. H Isolation and identification of Salmonella and Shigella. Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab.

Use isolates from appropriate media see Test Procedure. The bacteria thus isolated needs to be further identified to genus. Although it is often More information. The stones are made. Organisms capable of utilizing citrate produce alkaline metabolites which change in the color of the indicator from green acidic to deep blue alkaline. GRAM negative pathogens cocci and bacilli. In this investigation, you will study several More information. Standards Unit, Department More information.

Selective media allow certain types of organisms to grow, and inhibit the growth More information. Do not flame wire. Compare the different types of media. Welcome to Implementing Inquirybased Microbial Project.

Urease, an enzyme possessed by various microorganisms, hydrolyzes urea to ammonia causing the color of the indicator in the medium to shift from yellow acidic to red-purple alkaline. Explain the ratio of albumin and globulin More information.

Although it has been recently proposed to include Plesiomonas shigelloides into the family Enterobacteriaceae, this species is not included in the BBL Enterotube II databases. Professional English for Clinical Microbiology Unit 2: This group of bacteria utilizes carbohydrates principally by fermentation and by oxidation.


The medium remains yellow if decarboxylation of ornithine does not occur.

INSTRUCTIONS FOR USE BBL Enterotube II. IA Rev.: Jan. 2007

The medium remains yellow if decarboxylation of lysine does not occur. All organisms require a supply of energy and matter to build themselves and to continue to function. Identification of Unknown Organic Compounds Identification of Unknown Organic Compounds Introduction The identification and characterization of the structures of unknown substances are an important part of organic chemistry.

Cellular Respiration, Fermentation and Photosynthesis Introduction: Principle Urine cultures are performed to detect More information. It is a powerful oxidant. Energy of activation with and without an enzyme.

Daood Medical Microbiology Culture Media: The biochemical characteristics More information. Outer membrane of cell wall Lipid A What is it? Bacterial fermentation of adonitol, lactose, arabinose, and sorbitol result in the formation interpreetation acidic end products, is indicated by a change in color of the indicator present in the medium from red alkaline to yellow acidic. Ferric chloride need not to be added since the medium already contains an iron salt.


Name 10 routine chemical tests performed on urine and list a. Information on the appearance of positive and negative reactions is provided in Table 1. Describe the different formats of media, plate, tube etc.

Media, incubation, and aseptic technique Lab Exercise 3: BBL Enterotube II may be used to identify aerobic, facultatively anaerobic Gram negative rods Enterobacteriaceae isolated from any specimen.